isolation of adipose tissue stem cells with organ culture method

نویسندگان

ahmad ghorbani pharmacological research center of medicinal plants, faculty of medicine, mashhad university of medical sciences, iran.

seyed amir jalali immunogentics and tissue culture department, immunology research center, faculty of medicine, mashhad university of medical sciences, mashhad, iran.

masoumeh varedi department of physiology, faculty of medicine, shiraz university of medical sciences, shiraz, iran.

چکیده

background  isolation of adipose tissue mesenchymal stem cells (mscs) with current enzymatic methods has some limitations. for example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of mscs. also, it is accompanied with the increased risk of culture contaminations because of several handling steps.in this article we present a non-enzymatic method for isolation of mscs. methods  small pieces of rat adipose tissue and also human liposuction sample were placed in the culture flask, covered with fetal bovine serum (fbs) and maintained in incubator for 24 h. then, the fbs was changed with dmem medium containing 20% fbs. when the fibroblast-like cells were appeared around the tissue they were expanded through 3 passages and used for immunophenotype and differentiation assays. results  flow cytometric analysis showed that the cells isolated with organ culture method expressed cd44 and cd105 but did not express cd34 and cd45 markers. the isolated cells also differentiated into adipocyte and osteoblast.therefore, consistent with classically isolated mscs, the cells isolated with our method express the stem cell surface markers and have pluripotent property. conclusion  the presented method is an easy and cheap procedure and can be used for harvesting mscs from very small fat samples of human or animals. key words: adipocyte, human, osteoblast, stem cell.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation of Adipose Tissue Stem Cells with Organ Culture Method

Background  Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...

متن کامل

Isolation, Characterization and Differentiation of Rat Adipose Tissue Derived Mesenchymal Stem Cells

Introduction:   Mesenchymal stem cells have the potential of self-renewal and differentiation into different cell types, including blood cells, heart, nerves and cartilage, and have unlimited power for division. These cells can be obtained from cord, before implantation from fertilized cells and also from various tissues of adults although the differentiation power and the ability to reproduce ...

متن کامل

Evaluating the Viability of Adipose Tissue-derived Mesenchymal Stem Cells in Diabetic Culture Media Model

Introduction: In diabetes mellitus, stem cells are exposed to inappropriate conditions such as an increase of glucose in extracellular space, oxidative stress, and deprivation from growth factors (following ischemia). This study was aimed to evaluate the effect of these conditions on stem cells. Methods: Stem cells were isolated from subcutaneous adipose tissue of rats. The cells were maintaine...

متن کامل

Isolation, culture and identification of human adipose-derived stem cells.

The aim of the present study was to improve methods for the isolation and identification of adipose-derived stem cells (ASCs). Human subcutaneous adipose tissue was collected during liposuction surgery, without ultrasound-assisted liposuction and other assisted techniques, and digested with 0.075% collagenase I. First (P1) and second (P2) passage ASCs were applied to the subsequent experiments....

متن کامل

Isolation of stromal stem cells from human adipose tissue.

The stromal compartment of mesenchymal tissues is thought to harbor stem cells that display extensive proliferative capacity and multilineage potential. Stromal stem cells offer a potentially large therapeutic potential in the field of regenerative medicine. Adipose tissue contains a large number of stromal stem cells, is relatively easy to obtain in large quantities, and thus constitutes a ver...

متن کامل

isolation and culture of mesenchymal stem cells from rabbit scapular subcutaneous adipose tissue and their ability to differentiate into osteoblasts

conclusions based on our findings, we conclude that the separation and reproduction of adipose tissue cells is an appropriate method for purification of mscs in animal studies. regarding the histomorphometric and flow cytometry analysis results, we demonstrated the differentiation ability of mscs in normal medium and hope to employ these cells for the regeneration of damaged bone tissues in the...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of pediatrics

جلد ۲، شماره ۲.۳، صفحات ۵۳-۵۳

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023